This volume provides a detailed step-by-step description of protocols for the establishment of in vitro cultures of important medicinal plants, their mass multiplication in controlled environment, and step-wise secondary metabolite analysis. Many of these protocols also provide a basis for in vitro germplasm conservation or cryopreservation of medicinal plant species. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Protocols for In Vitro Cultures and Secondary Metabolite Analysis of Aromatic and Medicinal Plants, Second Edition aims to ensure successful results in the further study of this vital field.
1 Protocols for In Vitro Mass Multiplication and Analysis of Medicinally Important Phenolics of a Salep Orchid, Satyrium nepalense D.Don (“Salam Mishri”) - Shashi B. Babbar and Deepak K. Singh
2 In Vitro Culture and Phytochemical Analysis of Passiflora tenuifila Killip and Passiflora setacea DC (Passifloraceae) - Jenny Sumara Sozo, Daniel Cuzziol Cruz, Ana Flavia Pavei, Isadora Medeiros da Costa Pereira, Marcia Wolfart, Fernanda Ramlov, Daiane Fiuza Montagner, Marcelo Maraschin, and Ana Maria Viana
3 Shoot Tip Meristem Cryopreservation of Hypericum Species - Katarína Brunáková and Eva Cellárová
4 Protocols for Biotechnological Interventions in Improvement of Vanilla (Vanilla planifolia Andrews.) - Minoo Divakaran, K. Nirmal Babu, and K.V. Peter
5 Assessment of In Vitro Biological Activities of Anthocyanins-Rich Plant Species Based on Plinia cauliflora Study Model - Heloisa S. Pitz, Adriana C.D. Trevisan, Fausto R. Cardoso, Aline Pereira, Eduardo L.G. Moreira, Manuel A. de Prá, Letícia Mazzarino, Maria B. Veleirinho, Rosendo A. Yunes, Rosa M.R. do Valle, and Marcelo Maraschin
6 Biotechnological Approaches for Biomass and Cardenolide Production in Digitalis purpurea L. - Naivy Pérez-Alonso, Borys Chong-Pérez, Alina Capote, Anabel Pérez, André Gerth, Geert Angenon, and Elio Jiménez
7 In Vitro Regeneration of Endangered Medicinal Plant Heliotropium kotschyi (Ramram) - Manal Ahmed Sadeq, Malabika Roy Pathak, Ahmed Ali Salih, Mohammed Abido, and Asma Abahussain
8 Micropropagation and Biomass Production of True-to-Type Stevia rebaudiana Bertoni - Arpan R. Modi, Vikas Sharma, Ghanshyam Patil, Amritpal S. Singh, N. Subhash, and Nitish Kumar
9 Panax ginseng Adventitious Root Suspension Culture: Protocol for Biomass Production and Analysis of Ginsenosides by High Pressure Liquid Chromatography - Hosakatte Niranjana Murthy and Kee Yoeup Paek
10 Immobilization of Rubia tinctorum L. Suspension Cultures and Biomass Production - Pinar Nartop
11 In Vitro Propagation and Conservation of Bacopa monnieri L. - Neelam Sharma, Rakesh Singh, and Ruchira Pandey
12 Establishment, Culture, and Scale-up of Brugmansia candida Hairy Roots for the Production of Tropane Alkaloids - Alejandra Beatriz Cardillo, Julián Rodriguez Talou, and Ana María Giulietti
13 Micropropagation, Acclimatization, and Greenhouse Culture of Veratrum californicum - Sarah A. White, Jeffrey Adelberg, Jacqueline Naylor-Adelberg, David A. Mann, Ju Yeon Song, and Youping Sun
14 In Vitro Propagation of Withania somnifera (L.) Dunal - Pritika Singh, Rupam Guleri, and Pratap Kumar Pati
15 In Vitro Propagation of Sambong (Blumea balsamifera Linn.) - Thelma L. Soriano and Evangelina C. Cangao
16 Production of Gymnemic Acid from Cell Suspension Cultures of Gymnema sylvestre - Praveen Nagella, Vijayalaxmi S. Dandin, and Hosakatte Niranjana Murthy
17 Scale-Up of Agrobacterium rhizogenes-Mediated Hairy Root Cultures of Rauwolfia serpentina: ..